Anja Nohe
Assistant Professor
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Pre-Diplom (Chemistry)
University of Wuerzburg, Germany, 1993 |
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Diplom (Physical Chemistry) University of Wuerzburg, Germany, 1996 |
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Ph.D. (Physical Chemistry and Physiology) Theodor Boveri
Institute, Wuerzburg, Germany, 2000 |
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Research Interests
Image correlation
spectroscopy surface spectroscopy/microscopy bio-molecular
kinetics and dynamics signal transduction biological
membranes bone and joint development nano-particles
molecular diffusion
Certain domains and
regions on the cell membrane are thought to be important centers
for cell signaling, e.g. activation of signaling of receptors or
endocytosis of various proteins. These domains (e.g. caveolae,
rafts or coated pits) are enriched in different marker proteins
like caveolin-1, Ap-2 or GPI anchored proteins. My main interest
is focused on the distribution and aggregation of serine
threonine or tyrosine kinase receptors on the cell surface in
these domains and how this clustering and rearrangement of the
receptors by various stimuli can effect their signal
transduction pathways.
Image Correlation Spectroscopy (ICS) and Image
Cross-Correlation Spectroscopy (ICCS) are powerful tools to
study the distribution of membrane proteins. By labeling the
protein of interest fluorescently, taking high magnification
confocal images and implying ICS calculations it is possible to
get quantitative information about the average number of
clusters per unit area and the average number of proteins in
these clusters. By labeling proteins with different fluorescent
dyes it is possible by using ICCS to calculate the percent of
co-localization between these proteins.
Combining ICS and ICCS studies with standard molecular
biological tools, e.g. immunoprecipitations, western-blotting or
reporter gene assays, I try to get a better understanding of the
connection between the aggregation of receptors in specific
regions on the cell surface and the initiation of signal
transduction pathways.
A. Nohe, E.
Keating, T. M. Underhill, P. Knaus and N. O. Petersen. Dynamics
and interaction of caveolin-1 isoforms with BMP-receptors.
Accepted at J. of Cell Sci.
M. Kovar, A. Nohe, N. O. Petersen, P. Norton. Optical imaging
using NSOM. Photonics Handbook. 2004; Laurin Publishing,
H77-80.
A. Nohe and N. O. Petersen. Analyzing for co-localization of
proteins at a cell membrane. The
single way down from single genes, and proteins to single
molecules. Curr. Pharm Biotech. 2004; 5:213-220.
A. Nohe, E. Keating, C. Loh, M. T. Underhill, and N. O.
Petersen. Caveolin-1 isoform reorganization studied by Image
Correlation Spectroscopy. Faraday Discussion. 2004;
126:185-195.
A. Nohe, E. Keating, P. Knaus and N. O. Petersen. Signal
transduction of bone morphogenetic protein receptors. Cell.
Signal. 2004; 16:291-9.
S. Hassel, S. Schmitt, A. Hartung, M. Roth, A. Nohe, N.
Petersen, Y. Henis, W. Sebald and P. Knaus. Initiation of smad-dependent
and smad-independent signaling via distinct BMP-receptor
complexes. Journal of Bone and Joint Surgery. 2003;
85-A Suppl 3:44-51.
A. Nohe and N. O. Petersen. Analysing the protein-protein
interactions in cell membranes. Bioessays. 2003; 26:196-203.
A. Nohe, E. Keating, T. M. Underhill, P. Knaus and N. O.
Petersen. Effect of the distribution and clustering of the type
I A BMP receptor (ALK3) with the type II BMP receptor on the
activation of signalling pathways. J. of Cell Sci. 2003; 116:3277-3284.
F. Pohl, S. Hassel, A. Nohe, M. Flentje, P. Knaus, W. Sebald,
O. Koelbl. Radiation-induced suppression of the Bmp2 signal
transduction pathway in the pluripotent mesenchymal cell line
C2C12: An in vitro model for prevention of heterotopic
ossification by radiotherapy. Radiat Res. 2003 Mar;
159(3):345-50.
A. Nohe and N. O. Petersen. Image correlation spectroscopy:
Exploring intramolecular interactions. Biophotonics.
2002; 9:39-52.
A. Nohe, S. Hassel, M. Ehrlich, F. Neubauer, W. Sebald and P.
Knaus. The mode of BMP receptor oligomerization determines
different BMP-2 signaling pathways. J. Biol. Chem. 2002;
15:5330-5338.
L. Gilboa, A. Nohe, T. Geiίendφrfer, W. Sebald, Y. Henis and P.
Knaus. Bone morphogenetic protein receptor complexes on the
surface of live cells: A new oligomerization mode for serine/threonine
kinase receptors. Mol. Biol. Cell. 2000; 11:1023-1035.
A. Nohe, G. Lermann, H. Schwoerer, W. Kiefer, J. Sawatzki and
G. Surawicz. High resolution/low wavenumber Fourier transform
raman spectroscopy with a rubidium vapor filter and a
Ti:sapphire laser. J. Mol. Structure. 1997; 410-412:
65-68.
A. Nohe, G. Lermann, H. Schwoerer, W. Kiefer, J. Sawatzki and
G. Surawicz. High resolution/low wavenumber Fourier Transform
Raman Spectroscopy with a rubidium vapor filter and a Ti:
Sapphire laser. Proc. XVth Intern. Conf. Raman Spectroscopy, S.A.
Asher and P.B. Stein, Eds. 1996; Wiley, New York, 1240-1241. |
