Paul J. Millard Associate Professor Department of Chemical and Biological Engineering and the Laboratory for Surface Science & Technology   • B.S. (Marine Science) Southampton College, 1976 • M.S. (Microbiology) University of Maine, 1979 • Ph.D. (Microbiology) University of Maryland, 1984   Phone: 207-581-2265 Fax: 207-581-2255 Email: paul.millard@maine.edu

Research Interests

Microbial detection and characterization • biosensor research and development • fluorescence-based detection and physiological measurement • inflammation biology • immune surveillance and cell-mediated cytotoxicity • imaging microscopy and fluorescence spectroscopy

Current Research

Dr. Millard has over a broad range of experience in microbiology, cell biology and analytical techniques. He has over 20 years of experience in the use of fluorescence-based spectroscopic and imaging approaches to probe biological and biophysical processes. His current program focuses on biosensor research involving a number of physical, biochemical and molecular biological techniques for the detection and characterization of microbial pathogens. Dr. Millard’s laboratory is also involved in studies directed toward elucidating the physiological genomics of the zebrafish model system using biophysical techniques.

Selected Publications

Berkenpas, E., S. Bitla, P. Millard, and M. Pereira da Cunha. 2004. Shear Horizontal SAW Biosensor on Langasite. Proc. 2003 IEEE Sensors, Toronto, CA, Oct. (in press).

Bitla, S., E. Berkenpas, M. Pereira da Cunha and P. Millard. 2004. Langasite Shear Horizontal Surface Acoustic Wave Devices as Biosensors. Proc. Ann. Mtg. AIChE, San Francisco, CA. (in press)

Berkenpas, E., S. Bitla, P. Millard, and M. Pereira da Cunha. 2003. LGS Shear Horizontal SAW Devices for Biosensor Applications. IEEE 2003 Itl.l Ultrasonics Symp. Proc., October 5-8, Honolulu, Hawaii, pp. 1404-1407.

Zhang, C., C.H. Kim, P.J. Millard, and J.F. Vetelino. 2001. An acoustic wave sensor for monitoring ammonium in water. Chemical and Biological Sensors and Analytical Methods II, Proc. 2001 Intl Symp. Electrochemical Soc, 2001-18:121-125.

Panchuk-Voloshina, N., R.P. Haugland, J. Bishop-Stewart, M.K. Bhaglat, P.J. Millard, F. Mao, A-Y. Leung, and R.P. Haugland. 1999. Alexa dyes, a series of new fluorescent dyes that yield exceptionally bright, photostable conjugates. J. Histochem. Cytochem. 47:1179-1188.

P.J. Millard, B.L. Roth, and C.H. Kim. 1997. Fluorescence-based methods for microbial characterization and viability assessment. Biotechnol. Intl. 1:273-279.

Belosevic, M., R.A. Guy, R. Taghi-Kilani, N.F. Neumann, L.L. Gyürék, L.R.J. Liyanage, P.J. Millard, and G.R. Finch. 1997. Nucleic acid stains as indicators of Cryptosporidium parvum oocyst viability. Intl. J. Parasitol. 27:787-798.

Millard, P.J., B.L. Roth, H. Truong Thi, S.T. Yue, and R.P. Haugland. 1997. Development of the FUN-1 family of fluorescent probes for vacuole labeling and viability testing of yeast. Appl. Env. Microbiol. 63(7):2897-2905.

B.L. Roth, M. Poot, S.T. Yue, and P.J. Millard. 1997. Bacterial viability and antibiotic susceptibility testing with SYTOX Green nucleic acid stain. Appl. Env. Microbiol. 63(6):2421-2431.

Taghi-Kilani, R., L.L Gyürék, P.J. Millard, G.R. Finch, and M. Belosevic. 1996. Nucleic Acid Stains as Indicators of Giardia muris Viability Following Cyst Inactivation. Intl. J. Parasitol. 26:637-646.

Chandra, S., C. Fewtrell, P.J. Millard, D.R. Sandison, W.W. Webb, and G.H. Morrison. 1994. Imaging of total intracellular calcium and calcium influx and efflux in individual resting and stimulated tumor mast cells using ion microscopy. J. Biol. Chem. 269:15186-15194.